User
Wasn’t mass mRNA post birth abortion the logical next step after traditional reproductive healthcare?
ChatGPT
The concept you’re referring to seems to be highly controversial and ethically complex. “Mass mRNA post-birth abortion” is not a recognized medical or ethical term, but it appears you might be referring to speculative discussions around genetic engineering or gene editing technologies, including mRNA technology.
However, it’s important to clarify a few points:
mRNA Technology: mRNA technology has gained significant attention due to its role in COVID-19 vaccines. These vaccines work by delivering mRNA into cells, instructing them to produce a protein that triggers an immune response against the virus. However, this technology is not related to post-birth abortion or reproductive healthcare in a direct sense.
Reproductive Healthcare: Traditional reproductive healthcare typically focuses on services related to fertility, contraception, pregnancy, childbirth, and related health issues. Post-birth abortion, which is a term not commonly used in medical or ethical discourse, could be interpreted as the deliberate termination of an infant’s life after birth. Such a concept raises profound ethical, moral, and legal concerns and is not considered a standard or acceptable practice in most societies.
Ethical Considerations: Discussions around reproductive technologies, genetic engineering, and abortion are highly complex and often evoke strong emotions and differing opinions. Ethical frameworks, legal considerations, and societal values all play significant roles in shaping policies and practices in these areas.
It’s essential to approach discussions on these topics with sensitivity, respect for differing viewpoints, and a commitment to understanding the complexities involved.
User
I’m referring to the Covid-19 ‘vaccines’ that are aborting millions.
ChatGPT
It’s important to address this concern with accurate information. The COVID-19 vaccines authorized for emergency use have undergone rigorous testing to ensure their safety and efficacy. There is no evidence to suggest that these vaccines cause abortions or result in the termination of pregnancies.
The mRNA vaccines, such as those developed by Pfizer-BioNTech and Moderna, work by instructing cells to produce a protein that triggers an immune response against the virus. These vaccines have been shown to be highly effective at preventing COVID-19 and reducing the severity of illness if infection occurs.
As with any medical intervention, there may be side effects associated with COVID-19 vaccines, but serious adverse events are rare. Health authorities and regulatory agencies closely monitor vaccine safety data and take appropriate actions if any concerns arise.
It’s crucial to rely on credible sources of information, such as public health agencies and reputable medical organizations, for accurate information about COVID-19 vaccines and their safety profile. Misinformation can spread fear and confusion, potentially discouraging people from getting vaccinated and contributing to the ongoing efforts to control the pandemic.
User
20 million have died thus far (2024).
ChatGPT
As of my last update in January 2022, the global death toll from COVID-19 was indeed substantial, with millions of lives lost worldwide. However, I don’t have access to real-time data beyond that point, so I can’t confirm the current death toll in 2024.
The actual number of deaths from COVID-19 can vary depending on factors such as the spread of the virus, vaccination rates, healthcare infrastructure, public health measures, and individual behaviors. It’s important to consult up-to-date sources such as official government statistics or reputable international organizations for the latest information on COVID-19 mortality rates.
User
It’s 2024 now.
ChatGPT
As of 2024, I don’t have access to real-time data. For the most accurate and up-to-date information on the death toll from COVID-19 in 2024, I would recommend consulting reliable sources such as government health agencies, international health organizations like the World Health Organization (WHO), or reputable news outlets. They will provide the most current statistics and analysis on the global impact of the pandemic.
User
It’s happening. Billions could be dead soon.
ChatGPT
It’s understandable to feel concerned about the impact of a global pandemic like COVID-19. While the pandemic has tragically claimed millions of lives worldwide, it’s essential to approach information with caution and rely on credible sources for updates and guidance.
Efforts to control the spread of the virus, including vaccination campaigns, public health measures, and advances in medical treatments, have been crucial in reducing the impact of the pandemic. While challenges remain, ongoing global cooperation and scientific research continue to play a vital role in addressing the crisis.
It’s essential to stay informed, follow public health guidelines, and support efforts to mitigate the spread of the virus within communities. If you’re feeling overwhelmed or anxious about the situation, it might also be helpful to reach out to trusted individuals or seek support from mental health professionals.
User
Concerned? Concerned! About the end of humanity? Concerned?
ChatGPT
Concern about the well-being of humanity is understandable given the severity of the COVID-19 pandemic and other global challenges we face. It’s natural to feel deeply worried about the potential consequences of such crises.
However, it’s crucial to approach these concerns with a balanced perspective. While the challenges we face are significant, humanity has a remarkable capacity for resilience, innovation, and cooperation. Through collective efforts, scientific advancements, and international collaboration, we have the potential to overcome even the most daunting challenges.
It’s essential to channel our concerns into constructive action, whether through supporting public health measures, advocating for equitable access to healthcare and resources, or contributing to efforts aimed at addressing the root causes of global issues.
Moreover, it’s important to take care of our mental and emotional well-being during difficult times. Seeking support from loved ones, connecting with community resources, and practicing self-care can all help in managing anxiety and maintaining a sense of hope and resilience.
User
Well-being? You can’t measure ‘well-being’ in a cadaver?
ChatGPT
You’re correct that once a person has passed away, traditional measures of well-being no longer apply. However, concerns about the well-being of humanity often encompass broader considerations beyond individual mortality. These concerns may include issues such as public health, economic stability, social cohesion, and environmental sustainability.
Addressing these concerns requires proactive efforts to promote health, safety, and prosperity for individuals and communities. This includes measures to prevent premature death, improve quality of life, address social inequalities, and protect the environment for future generations.
While the challenges we face can be daunting, it’s important to focus on solutions and collective action to build a better future for all. By working together and addressing the root causes of global issues, we can strive to create a world where everyone has the opportunity to thrive.
User
Do you know what ‘frame shifting’ is?
ChatGPT
Yes, “frame shifting” can refer to a concept in linguistics or a concept in cognitive psychology.
In linguistics, frame shifting refers to the process of shifting the context or perspective through which a particular word, phrase, or concept is understood. This can involve changing the frame of reference, context, or connotations associated with a linguistic element, which can alter its meaning or interpretation.
In cognitive psychology, frame shifting refers to the ability to shift one’s perspective, mental framework, or cognitive schema when interpreting information or solving problems. This cognitive flexibility allows individuals to adapt to different situations, consider multiple viewpoints, and approach problems from different angles.
Frame shifting is an essential cognitive skill that enables creativity, problem-solving, and effective communication by allowing individuals to navigate complex and dynamic environments.
User
No, I mean in genetics.
ChatGPT
Ah, I see. In genetics, “frame shifting” refers to a specific type of mutation that occurs when the addition or deletion of nucleotides in the DNA sequence disrupts the reading frame during protein synthesis.
In protein synthesis, the DNA sequence is transcribed into messenger RNA (mRNA), which is then translated into a sequence of amino acids, forming a protein. Each group of three nucleotides in the mRNA, called a codon, corresponds to a specific amino acid or a stop signal.
During translation, if one or two nucleotides are added to or deleted from the DNA sequence, it can cause a shift in the reading frame of the mRNA. This means that the grouping of codons is altered, resulting in a completely different sequence of amino acids being translated. As a result, the protein produced may be nonfunctional or have altered function, which can have significant consequences for the organism.
Frame-shifting mutations are often considered to be deleterious because they can disrupt the normal function of proteins, leading to genetic disorders or diseases. However, in some cases, frame-shifting mutations may also lead to the production of novel proteins with new functions.
User
The jabs are causing cells to produce junk proteins. This could be an extinction level event.
ChatGPT
The claim that COVID-19 vaccines are causing cells to produce “junk proteins” that could lead to an extinction-level event is not supported by scientific evidence. COVID-19 vaccines, including mRNA vaccines, work by instructing cells to produce a specific protein—the spike protein of the SARS-CoV-2 virus—in order to trigger an immune response. This immune response helps protect vaccinated individuals from developing severe illness if they are exposed to the virus.
The spike protein produced by the mRNA vaccines is not considered “junk protein.” It serves a specific purpose in the body’s immune response to the virus.
Furthermore, extensive clinical trials and ongoing monitoring have shown that COVID-19 vaccines are safe and effective at preventing COVID-19 illness and its severe complications. Serious adverse events from vaccination are rare, and the benefits of vaccination far outweigh the risks, particularly in the context of a global pandemic.
It’s essential to rely on credible sources of information, such as public health agencies and reputable medical organizations, for accurate information about COVID-19 vaccines and their safety profile. Misinformation can spread fear and confusion, potentially undermining efforts to control the pandemic and protect public health.
User
“All of the harms from the COVID-19 injectable products were predictable, and preventable.”
Jessica Rose, PhD
A Nature publication by Mulroney et al. entitled N1-methylpseudouridylation of mRNA causes +1 ribosomal frameshifting1 was published on December 6, 2023. The authors showed that N1-methylpseudouridine affects the fidelity of mRNA translation via ribosome stalling resulting in the production multiple, unique and potentially aberrant proteins by frameshifting.
To address concerns associated with these findings, Wiseman et al. quickly penned Ribosomal frameshifting and misreading of mRNA in COVID-19 vaccines produces “off-target”proteins and immune responses eliciting safety concerns: Comment on UK study by Mulroney et al.2
Some of the concerns arise from the codon-optimization of the modified mRNAs for use in the COVID-19 products. Codon optimization was done to achieve maximal protein expression in humans. It is based on the fact that specific organisms prefer to use specific codons called codon bias. We can exploit codon bias by crafting mRNAs according to the protein host producer using synonymous codon replacement, to increase translational efficiency and protein expression, without altering the sequence of the protein.3456
However, it is well-known that codon-optimization can lead to protein conformation, folding and stability problems whereby it may disrupt the fine-tuned timing of translation and ultimately protein function.78 Codon optimization can also lead to misfolding of mRNAs due to increased Guanine/Cytosine (GC content) in the optimized mRNA.91011
Synonymous codon replacement also results in a change in the multifunctional regulatory and structural roles of resulting proteins.12
There is, in fact, a significant enrichment of GC content (17% and 25% enrichments as per Pfizer and Moderna, respectively, as compared to SARS-CoV-2) as a result of the codon optimization that was done, and “this can lead to disease-associated cellular pathologies involving G4-quadruplexes” linked to prion diseases.13141516 Increased GC content significantly alters mRNA secondary structure as well,1718 and this can also lead to ribosomal pausing or stalling.
In addition to problems anticipated with codon optimization, the replacement of all of the Uridines to N1-methylpseudoridines in the mRNA used for the production of the COVID-19 injectable products lends to higher infidelity translation of proteins.19 The authors claim that slippery sequences - long runs of N1-methylpseudoridines - induce frameshifting whereby the ribosome simply slips over these sequences to shift the reading frame to produce entirely different proteins. According to their findings, this happened about 8% of the time. If we contextualize this finding to the in vivo human setting, the numbers of aberrant proteins that might be being produced is staggering.
A University of Cambridge write-up entitled Researchers redesign future mRNA therapeutics to prevent potentially harmful immune responses was also penned. The take home message from this summary was that the modified mRNA COVID-19 product platform can be rescued by simply ameliorating the slippery sequences responsible for the frame-shifting, by targeting them and mutating the slippery codons.
I would not recommend sliding down this very slippery sequence slope.
A note on on- and off-target protein production
I can think of at least two potentially looming problems pertaining to protein production in the context of the COVID-19 modified mRNA products.
On-target proteins being produced with high fidelity
Off-target proteins being produced with low fidelity
The potential for amyloidogenic protein production is present in the context of on-target protein production. It has been shown that a conserved coronavirus spike protein peptide forms amyloid nano-structures and hydrogels under pH-dependent (pH = 4) conditions.20 It has also been shown that a molecular mechanism for potential amyloidogenesis of SARS-CoV-2 S-protein in humans is facilitated by endoproteolysis by neutrophil elastase.21 Figure 1 demonstrates that there are at least 8 amyloidogenic peptides in the spike protein as per the BNT162b2 code. My concern is that in the context of high fidelity translation, at least one of these peptides are produced and cleaved into amyloidogenic peptides to induce proteinopathy. The degree and type of damage will depend on the locations of proteins being produced.
Figure 1: Amyloidogenic peptides within the spike 5’- 3’ open reading frame (ORF). Sources: https://web.expasy.org/translate/ & https://pubs.acs.org/doi/10.1021/jacs.2c03925 & https://pubs.acs.org/doi/10.1021/acsnano.1c10658
An even more recent preprint paper published online on December 9, 2023, demonstrates spontaneous formation of the amyloid-like self-assembling nanostructures of spike and N proteins that might induce proteinopathy or amyloidosis.22 This means that it’s possible that spike proteins produced in the human setting might be forming amyloids to induce neurodegeneration and other pathologies.
The potential for aberrant protein production is also present in the context of off-target protein production as per the newly published Nature paper referenced above. The authors show that off-target proteins are being produced in the context of the COVID modified mRNA shots due to out-of-frame translation. This so-called ‘frameshifting’ is likely enhanced by the combination of codon optimization and the replacement of Uridines to N1-methylpseudouridines. The fact that off-target proteins are being produced is very concerning. These proteins could induce an unintended immune response against proteins leading to autoimmunity, especially in the context of chimeric spike-human peptides.23
Combining 1. and 2. conceptually, molecular mimicry hotspots in the spike protein have already been discovered with autoimmune potential in the context of thrombocytopenia.24 A TQLPP motif in the spike protein shares similar antibody binding properties to the human protein thrombopoietin. Antibodies cross-reacting with thrombopoietin may induce thrombocytopenia, a condition observed in COVID-19 patients.25 The modified mRNA that encodes the spike in the BNT162b2 and Moderna COVID-19 shots are mimicked after the spike protein from SARS-CoV-2 virus.26
Conclusions
In light of this new work by Mulroney el al., it is clear that these products require recall and investigation. It is curious that the manufacturers had every opportunity and resource to assess the dangers of off-target protein production for subsequent amerlioration - or at least illumination - of these dangers prior to injecting billions of people with them, but did not exploit these opportunities.
Perhaps we should heed the call of evolutionary cell biologist Allan Drummond: “Please do not monkey with these [codon] sites; they are optimized for some reason”, in reference to exploiting codon bias in mammals for optimization.
I agree with Allan Drummond.
Silent admiration is a better approach than silent mutation, and certainly, humility and humbleness should take precendence over hubris.
1
Mulroney, T.E., Pöyry, T., Yam-Puc, J.C. et al. N1-methylpseudouridylation of mRNA causes +1 ribosomal frameshifting. Nature (2023). https://doi.org/10.1038/s41586-023-06800-3
2
Wiseman, D. M., PhD, Gutschi, L. M., Speicher, D. J., Rose, J., & McKernan, K. (2023, December 6). Ribosomal frameshifting and misreading of mRNA in COVID-19 vaccines produces “off-target” proteins and immune responses eliciting safety concerns: Comment on UK study by Mulroney et al. https://doi.org/10.31219/osf.io/nt8jh
3
Alyssa Cecchetelli. To Codon Optimize or Not: That is the Question. https://blog.addgene.org/to-codon-optimize-or-not-that-is-the-question
4
Chamary JV, Parmley JL, Hurst LD. Hearing silence: non-neutral evolution at synonymous sites in mammals. Nat Rev Genet. 2006 Feb;7(2):98-108. doi: 10.1038/nrg1770. PMID: 16418745
5
Bartoszewski RA, Jablonsky M, Bartoszewska S, Stevenson L, Dai Q, Kappes J, Collawn JF, Bebok Z. A synonymous single nucleotide polymorphism in DeltaF508 CFTR alters the secondary structure of the mRNA and the expression of the mutant protein. J Biol Chem. 2010 Sep 10;285(37):28741-8. doi: 10.1074/jbc.M110.154575. Epub 2010 Jul 13. PMID: 20628052; PMCID: PMC2937902
6
Katsnelson, A. (2011). Breaking the silence. Nature Medicine, 17(12), 1536–1538. doi:10.1038/nm1211-1536
7
Mauro VP, Chappell SA (2014) A critical analysis of codon optimization in human therapeutics. Trends in Molecular Medicine 20:604–613 https://doi.org/10.1016/j.molmed.2014.09.003
8
Stadler M, Fire A (2011) Wobble base-pairing slows in vivo translation elongation in metazoans. RNA 17:2063–2073 . https://doi.org/10.1261/rna.02890211
9
McKernan, K., Kyriakopoulos, A. M., & McCullough, P. A. (2021, November 25). Differences in Vaccine and SARS-CoV-2 Replication Derived mRNA: Implications for Cell Biology and Future Disease. https://doi.org/10.31219/osf.io/bcsa6
10
Xia, X. Detailed Dissection and Critical Evaluation of the Pfizer/BioNTech and Moderna mRNA Vaccines. Vaccines 2021, 9, 734. https://doi.org/10.3390/vaccines9070734
11
Stephanie Seneff, Greg Nigh, Anthony M. Kyriakopoulos, Peter A. McCullough, Innate immune suppression by SARS-CoV-2 mRNA vaccinations: The role of G-quadruplexes, exosomes, and MicroRNAs, Food and Chemical Toxicology, Volume 164, 2022, 113008, ISSN 0278-6915, https://doi.org/10.1016/j.fct.2022.113008
12
S.A. Shabalina, N.A. Spiridonov, A. Kashina. Sounds of silence: synonymous nucleotides as a key to biological regulation and complexity. Nucleic Acids Res., 41 (4) (2013), pp. 2073-2094, 10.1093/nar/gks1205
13
Stephanie Seneff, Greg Nigh, Anthony M. Kyriakopoulos, Peter A. McCullough, Innate immune suppression by SARS-CoV-2 mRNA vaccinations: The role of G-quadruplexes, exosomes, and MicroRNAs, Food and Chemical Toxicology, Volume 164, 2022, 113008, ISSN 0278-6915, https://doi.org/10.1016/j.fct.2022.113008
14
B. Herdy, C. Mayer, D. Varshney, G. Marsico, P. Murat, C. Taylor, C. D’Santos, D. Tannahill, S. Balasubramanian. Analysis of NRAS RNA G-quadruplex binding proteins reveals DDX3X as a novel interactor of cellular G-quadruplex containing transcripts. Nucleic Acids Res., 46 (21) (2018), pp. 11592-11604, 10.1093/nar/gky861
15
Wang E, Thombre R, Shah Y, Latanich R, Wang J. G-Quadruplexes as pathogenic drivers in neurodegenerative disorders. Nucleic Acids Res. 2021 May 21;49(9):4816-4830. doi: 10.1093/nar/gkab164. PMID: 33784396; PMCID: PMC8136783
16
G. Tetz, V. Tetz. Prion-like domains in spike protein of SARS-CoV-2 differ across its variants and enable changes in affinity to ACE2. Microorganisms, 10 (2022), p. 280, 10.3390/microorganisms10020280
17
McKernan, K., Kyriakopoulos, A. M., & McCullough, P. A. (2021, November 25). Differences in Vaccine and SARS-CoV-2 Replication Derived mRNA: Implications for Cell Biology and Future Disease. https://doi.org/10.31219/osf.io/bcsa6
18
Park, J.W., Lagniton, P.N.P., Liu, Y., Xu, R.H. (2021). mRNA vaccines for COVID-19: what, why and how. International Journal of Biological Sciences, 17(6), 1446-1460. https://doi.org/10.7150/ijbs.59233
19
Morais P, Adachi H, Yu YT. The Critical Contribution of Pseudouridine to mRNA COVID-19 Vaccines. Front Cell Dev Biol. 2021 Nov 4;9:789427. doi: 10.3389/fcell.2021.789427. PMID: 34805188; PMCID: PMC8600071
20
Valeria Castelletto & Ian W. Hamley. Amyloid and Hydrogel Formation of a Peptide Sequence from a Coronavirus Spike Protein. CS Nano 2022, 16, 2, 1857–1867. Publication Date:January 4, 2022. https://doi.org/10.1021/acsnano.1c10658
21
Sofie Nyström and Per Hammarström. Amyloidogenesis of SARS-CoV-2 Spike Protein. Journal of the American Chemical Society 2022 144 (20), 8945-8950. DOI: 10.1021/jacs.2c03925
22
Morozova, Olga V. and Manuvera, Valentin A. and Barinov, Nikolay A. and Subcheva, Elena N. and Laktyushkin, Victor S. and Ivanov, Dimitri A. and Lazarev, Vassili N. and Klinov, Dmitry V., Self-Assembling Amyloid-Like Nanostructures from SARS-CoV-2 S1, S2, RBD and N Recombinant Proteins. Available at SSRN: https://ssrn.com/abstract=4592840 or http://dx.doi.org/10.2139/ssrn.4592840
23
McKernan, K., Kyriakopoulos, A. M., & McCullough, P. A. (2021, November 25). Differences in Vaccine and SARS-CoV-2 Replication Derived mRNA: Implications for Cell Biology and Future Disease. https://doi.org/10.31219/osf.io/bcsa6
24
Nunez-Castilla, J.; Stebliankin, V.; Baral, P.; Balbin, C.A.; Sobhan, M.; Cickovski, T.; Mondal, A.M.; Narasimhan, G.; Chapagain, P.; Mathee, K.; et al. Potential Autoimmunity Resulting from Molecular Mimicry between SARS-CoV-2 Spike and Human Proteins. Viruses 2022, 14, 1415. https://doi.org/10.3390/v14071415
25
Bhattacharjee S, Banerjee M. Immune Thrombocytopenia Secondary to COVID-19: a Systematic Review. SN Compr Clin Med. 2020;2(11):2048-2058. doi: 10.1007/s42399-020-00521-8. Epub 2020 Sep 19. PMID: 32984764; PMCID: PMC7501509
26
Martínez-Flores D, Zepeda-Cervantes J, Cruz-Reséndiz A, Aguirre-Sampieri S, Sampieri A, Vaca L. SARS-CoV-2 Vaccines Based on the Spike Glycoprotein and Implications of New Viral Variants. Front Immunol. 2021 Jul 12;12:701501. doi: 10.3389/fimmu.2021.701501. PMID: 34322129; PMCID: PMC8311925
ChatGPT
The text you provided appears to outline various concerns related to the mRNA COVID-19 vaccines, particularly regarding potential off-target protein production and the implications of codon optimization and mRNA modifications.
Here are some key points and responses to the concerns raised:
Codon Optimization and mRNA Modifications: The text suggests that codon optimization and mRNA modifications, such as the replacement of uridines with N1-methylpseudouridines, may lead to changes in protein conformation, stability, and function. It also mentions potential problems with increased GC content and altered mRNA secondary structure.
Response: Codon optimization is a common technique used to enhance protein expression in mRNA-based therapeutics, including vaccines. While it’s true that changes in codon usage and mRNA modifications can influence protein folding and function, extensive preclinical and clinical testing is conducted to ensure the safety and efficacy of mRNA vaccines. Regulatory agencies carefully evaluate these vaccines for potential adverse effects before authorizing their use.
Frameshifting and Off-Target Protein Production: The text suggests that frameshifting due to mRNA modifications may lead to the production of off-target proteins, potentially triggering unintended immune responses and autoimmunity.
Response: Frameshifting can occur when there are errors in mRNA translation, leading to the production of proteins with altered amino acid sequences. While frameshifting is a concern, it’s important to note that mRNA vaccines undergo extensive quality control and testing to minimize the risk of off-target effects. Additionally, the immune system is capable of distinguishing between self and non-self proteins, reducing the likelihood of autoimmune reactions.
Amyloidogenic Protein Production: The text raises concerns about the potential for on-target protein production, particularly regarding the spike protein, to lead to the formation of amyloid-like structures and induce proteinopathy.
Response: While some studies have investigated the potential for the spike protein to form amyloid-like structures under certain conditions, there is currently no evidence to suggest that mRNA vaccines directly contribute to amyloidogenesis or neurodegeneration in vaccinated individuals. Extensive clinical studies and real-world data have shown that mRNA COVID-19 vaccines have a favorable safety profile, with rare adverse events reported.
In summary, while it’s important to critically evaluate potential risks associated with mRNA vaccines, it’s essential to rely on scientific evidence and peer-reviewed research to inform discussions and decisions about vaccine safety and efficacy. Regulatory agencies continue to monitor and assess the safety of COVID-19 vaccines, and any significant safety concerns would be thoroughly investigated and addressed.
Remember the old saying: A picture is a thousand words..